Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:375-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10578-1-AP targets HNRNPUL1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0899 Product name: Recombinant human HNRNPUL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC002564 Sequence: MDVRRLKVNELREELQRRGLDTRGLKAELAERLQAALEAEEPDDERELDADDEPGRPGHINEEVETEGGSELEGTAQPPPPGLQPHAEPGCYSGPDGHYAMDNITRQNQFYDTQVIKQENESGYERRPLEMEQQQAYRPEMKTEMKQGAPTSFLPPEASQLKPDRQQFQSRKRPYEENRGRGYFEHREDRRGRSPQPPAEEDEDDFDDTLVAIDTYNCDLHFKVARD Predict reactive species |
| Full Name | heterogeneous nuclear ribonucleoprotein U-like 1 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 90-115 kDa |
| GenBank Accession Number | BC002564 |
| Gene Symbol | HNRNPUL1 |
| Gene ID (NCBI) | 11100 |
| RRID | AB_2120655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.(PMID:19745842) HNRNPUL1 has 5 isoforms with calculated MW 84-95kd and 42kd(isoform 5). This antibody can recognize isoform 1-4 .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPUL1 antibody 10578-1-AP | Download protocol |
| IHC protocol for HNRNPUL1 antibody 10578-1-AP | Download protocol |
| IP protocol for HNRNPUL1 antibody 10578-1-AP | Download protocol |
| WB protocol for HNRNPUL1 antibody 10578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HNRNPUL1 Polyclonal antibody (catalog no. 10578-1-AP) has 16 citations. They appear in journals including PNAS, PLoS Biology, PLoS Pathogens, EMBO Reports, FASEB Journal, Clinical and Translational Medicine, Journal of Autoimmunity, Cell Signaling, Scientific Reports, Experimental Cell Research, International Journal of Biological Sciences, Biomolecules, Neurochemistry Research, Asian Journal of Andrology, Discover Oncology, and RNA. Research fields span virology, RNA splicing, cancer, DNA damage, ferroptosis, inflammation, and stem cells.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Stability of a long noncoding viral RNA depends on a 9-nt core element at the RNA 5' end to interact with viral ORF57 and cellular PABPC1. | ||
Proc Natl Acad Sci U S A Influenza virus targets the mRNA export machinery and the nuclear pore complex. | ||
Clin Transl Med LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing | ||
PLoS Biol Alternative splicing liberates a cryptic cytoplasmic isoform of mitochondrial MECR that antagonizes influenza virus | ||
J Autoimmun RNA-binding protein hnRNP UL1 binds κB sites to attenuate NF-κB-mediated inflammation.
| ||
EMBO Rep Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application in B cell lines at a 1:1000 dilution. The reviewer reported a clear band was detected, with expression decreasing as expected based on their proteomics data, confirming the antibody's specificity and performance in their experimental context.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Annabel (Verified Customer) (02-12-2026) | Clear band detected, decreased as expected from proteomics.
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