HOMER1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85692-4-RR (same clone as 85692-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85692-4-RR (same clone as 85692-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  IF Staining of MOLT-4 using 85692-4-RR (same clone as 85692-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using HOMER1 antibody (85692-4-RR, Clone: 243007G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85692-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85692-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

HOMER1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 85692-4-PBS

Clone No.243007G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

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Product Information

85692-4-PBS targets HOMER1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3109

  Product name: Recombinant human HOMER1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 7-354 aa of BC015502

  Sequence: FSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHTHKTELNQTIQELEETLKLKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSDLEQRLEKSQNEQEAFRNNLKTLLEILDGKIFELTELRDNLAKLLECS

• Full Name: homer homolog 1 (Drosophila)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 46-48 kDa
• GenBank Accession Number: BC015502
• Gene Symbol: HOMER1
• Gene ID (NCBI): 9456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86YM7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.