HOMER2 Monoclonal antibody

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  WB analysis of LNCaP using 67377-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67377-1-Ig (HOMER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67377-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67377-1-Ig (HOMER2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 67377-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67377-1-Ig (HOMER2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 67377-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67377-1-Ig (HOMER2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HOMER2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67377-1-Ig

Clone No.1G5G1

Host / Isotype

Mouse / IgG3

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

ACPD, CPD, Cupidin, HOMER 2, homer homolog 2 (Drosophila), Homer protein homolog 2, HOMER2, HOMER2A, HOMER2B, Vesl 2

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67377-1-Ig targets HOMER2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25177

  Product name: Recombinant human HOMER2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 45-343 aa of BC012109

  Sequence: YRIISVDGAKVIINSTITPNMTFTKTSQKFGQWADSRANTVFGLGFSSEQQLTKFAEKFQEVKEAAKIAKDKTQEKIETSSNHSQASSVNGTDDEKASHAGPANTHLKSENDKLKIALTQSAANVKKWEIELQTLRESNARLTTALQESAASVEQWKRQFSICRDENDRLRNKIDELEEQCSEINREKEKNTQLKRRIEELEAELREKETELKDLRKQSEIIPQLMSECEYVSEKLEAAERDNQNLEDKVRSLKTDIEESKYRQRHLKVELKSFLEVLDGKIDDLHDFRRGLSKLGTDN

• Full Name: homer homolog 2 (Drosophila)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC012109
• Gene Symbol: HOMER2
• Gene ID (NCBI): 9455
• RRID: AB_2882624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NSB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.