HOOK2 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• HOOK2 Polyclonal antibody (12458-1-AP)

HOOK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12458-1-AP Copied!

Publications (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

h hook2, hHK2, h-hook2, HK2, hook homolog 2 (Drosophila)

• 

  WB analysis using 12458-1-AP

  SH-SY5Y cell lysates were subjected to SDS PAGE followed by western blot with 12458-1-AP (HOOK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 12458-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12458-1-AP (HOOK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 12458-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HOOK2 antibody (12458-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for HOOK2 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12458-1-AP targets HOOK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3124

  Product name: Recombinant human HOOK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC012443

  Sequence: MSVDKAELCGSLLTWLQTFHVPSPCASPQDLSSGLAVAYVLNQIDPSWFNEAWLQGISEDPGPNWKLKVSNLKMVLRSLVEYSQDVLAHPVSEEHLPDVSLIGEFSDPAELGKLLQLVLGCAISCEKKQDHIQRIMTLEESVQHVVMEAIQELMTKDTPDSLSPETYGNFDSQSRRYYFLSEEAEEGDELQQRCLDLERQLMLLSEEKQSLAQENAGLRERMGRPEGEGTPGLTAKKLLLLQSQLEQLQEENFRLESGREDERLRCAELEREVAELQHRNQALTSLAQEAQALKDEMDELRQSSERAGQLEATLTSCRRRLGELRELRRQVRQLEERNAGHAERTRQLED

• Full Name: hook homolog 2 (Drosophila)
• Calculated Molecular Weight: 717 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC012443
• Gene Symbol: HOOK2
• Gene ID (NCBI): 29911
• RRID: AB_3669139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96ED9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The HOOK2 Polyclonal antibody (Cat# 12458-1-AP) has 1 citation. It was cited in Journal of Ovarian Research (IF 5.3, 2025), in a study on modified screening of MYC promoter region elements using CRISPR library in ovarian cancer. The research field is oncology, specifically ovarian cancer genetics and epigenetic regulation. The antibody was used for Western blot in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Ovarian Res; Modified screening of MYC promotor region elements using the CRISPR library in ovarian cancer; Authors - Akiko Yamamoto