HOOK3 Polyclonal antibody, PBS Only

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• HOOK3 Polyclonal antibody, PBS Only (15457-1-PBS)

• 

  WB analysis of rat liver using 15457-1-AP (same clone as 15457-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 15457-1-AP (same clone as 15457-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 15457-1-AP (same clone as 15457-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 15457-1-AP (same clone as 15457-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 15457-1-AP (same clone as 15457-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 15457-1-AP (same clone as 15457-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 15457-1-AP (same clone as 15457-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15457-1-AP (HOOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 15457-1-AP (same clone as 15457-1-PBS)

  IP result of anti-HOOK3 (IP:15457-1-AP, 4ug; Detection:15457-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15457-1-AP (same clone as 15457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15457-1-AP (same clone as 15457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15457-1-AP (HOOK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 15457-1-AP (same clone as 15457-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15457-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HOOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15457-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

15457-1-PBS targets HOOK3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7724

  Product name: Recombinant human HOOK3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 370-718 aa of BC056146

  Sequence: TYKRQVVELQNRLSEESKKADKLDFEYKRLKEKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSSRRSYPGHVQPATAR

• Full Name: hook homolog 3 (Drosophila)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC056146
• Gene Symbol: HOOK3
• Gene ID (NCBI): 84376
• RRID: AB_2120828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VS8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.