HORMAD1 Polyclonal antibody

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• HORMAD1 Polyclonal antibody (13917-1-AP)

HORMAD1 Polyclonal Antibody for WB, ELISA

Cat No. 13917-1-AP Copied!

Publications (34)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, CoIP, ELISA

Synonyms

CT46, HORMA domain-containing protein 1, HORMAD 1, NOHMA

• 

  WB analysis using 13917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13917-1-AP (HORMAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 13917-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13917-1-AP (HORMAD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-468 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13917-1-AP targets HORMAD1 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4914

  Product name: Recombinant human HORMAD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-394 aa of BC047406

  Sequence: MATAQLQRTPMSALVFPNKISTEHQSLVLVKRLLAVSVSCITYLRGIFPECAYGTRYLDDLCVKILREDKNCPGSTQLVKWMLGCYDALQKKYLRMVVLAVYTNPEDPQTISECYQFKFKYTNNGPLMDFISKNQSNESSMLSTDTKKASILLIRKIYILMQNLGPLPNDVCLTMKLFYYDEVTPPDYQPPGFKDGDCEGVIFEGEPMYLNVGEVSTPFHIFKVKVTTERERMENIDSTILSPKQIKTPFQKILRDKDVEDEQEHYTSDDLDIETKMEEQEKNPASSELEEPSLVCEEDEIMRSKESPDLSISHSQVEQLVNKTSELDMSESKTRSGKVFQNKMANGNQPVKSSKENRKRSQHESGRIVLHHFDSSSQESVPKRRKFSEPKEHI

• Full Name: HORMA domain containing 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC047406
• Gene Symbol: HORMAD1
• Gene ID (NCBI): 84072
• RRID: AB_2120844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86X24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734]. HORMAD1 exists as various isoform and the range of molecular weight of isoforms are sbout 36-45 kDa.

Publications

What published studies show

The anti-HORMAD1 antibody (Cat# 13917-1-AP) has 34 citations across 27 journals, including Nature, Sci Adv, EMBO J, Cell Reports, Nucleic Acids Research, Development, and PLoS Genetics. Research fields span meiosis (spermatogenesis, synapsis, recombination), chromosome biology, DNA damage repair, cancer (gastric cancer, T-cell lymphoma), and stem cell biology. Applications include WB, IF, and CoIP in mouse and human systems.

Automatically generated from the 34 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease; Authors - Tae-Yoon Park
		Nature; SIRT7 regulates dosage compensation and safeguards the female X chromosome.; Authors - Nicolas G. Simonet
mouse	IF	Exp Mol Med; Structured illumination microscopy imaging reveals localization of replication protein A between chromosome lateral elements during mammalian meiosis.; Authors - Seobin Yoon
mouse,human	WB,IF,CoIP	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
mouse	IF	Adv Sci (Weinh); m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control; Authors - Lisha Yin