Tested Applications
| Positive WB detected in | Jurkat cells, SKOV-3 cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22256-1-AP targets HOXB4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17761 Product name: Recombinant human HOXB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-72 aa of BC049204 Sequence: EYSQSDYLPSDHSPGYYAGGQRRESSFQPEAGFGRRAACTVQRYAACRDPG Predict reactive species |
| Full Name | homeobox B4 |
| Calculated Molecular Weight | 251 aa, 28 kDa |
| Observed Molecular Weight | 28-35 kDa |
| GenBank Accession Number | BC049204 |
| Gene Symbol | HOXB4 |
| Gene ID (NCBI) | 3214 |
| RRID | AB_2879050 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17483 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXB4 antibody 22256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HOXB4 Polyclonal antibody (Cat# 22256-1-AP) has 1 citation to date. It was cited in Molecular Carcinogenesis (2025), in a study investigating how circ_0044362 promotes epithelial ovarian cancer progression by enhancing HOXB4 transcription to activate the RUNX1/IGFBP3 axis. The research field is oncology, specifically ovarian cancer molecular mechanisms and circRNA-mediated gene regulation. The antibody was used for Western blot in both human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Carcinog Circ_0044362 Facilitates the Progression of Epithelial Ovarian Cancer via Enhancing HOXB4 Transcription to Activate the RUNX1/IGFBP3 Axis
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