HOXC10 Polyclonal antibody

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  WB analysis of mouse heart using 12025-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12025-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12025-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 12025-1-AP (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 12025-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12025-1-AP (HOXC10 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HOXC10 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 12025-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I

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Tested Applications

• Positive WB detected in: mouse heart tissue, A549 cells, C2C12 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

12025-1-AP targets HOXC10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2655

  Product name: Recombinant human HOXC10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-342 aa of BC001293

  Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTGNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKTINLTDRQVKIWFQNRRMKLKKMNRENRIRELTSNFNFT

• Full Name: homeobox C10
• Calculated Molecular Weight: 342 aa, 38 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC001293
• Gene Symbol: HOXC10
• Gene ID (NCBI): 3226
• RRID: AB_2119611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYD6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Int Immunopharmacol; HOXC6/8/10/13 predict poor prognosis and associate with immune infiltrations in glioblastoma.; Authors - Mingjun Yu
human	WB,IHC	Chin J Cancer Res; HOXC10 up-regulation promotes gastric cancer cell proliferation and metastasis through MAPK pathway.; Authors - Chen Guo
human	WB,IF	Cell Rep; Identification of cis-HOX-HOXC10 axis as a therapeutic target for colorectal tumor-initiating cells without APC mutations.; Authors - Zhenzhen Chen ; KO Validated