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HOXC10 Recombinant monoclonal antibody, PBS Only (86219-3-PBS)

HOXC10 Recombinant monoclonal antibody, PBS Only

Name: HOXC10 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 86219-3-PBS-100UG

HOXC10 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86219-3-PBS

Clone No.250873D8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Homeobox protein Hox-3I, Homeobox protein Hox-C10, HOX3I

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBIF/ICCAffinity

WB analysis using 86219-3-RR (same clone as 86219-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86219-3-RR (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86219-3-PBS in a different storage buffer formulation.
WB analysis using 86219-3-RR (same clone as 86219-3-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86219-3-RR (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86219-3-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 86219-3-RR (same clone as 86219-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXC10 antibody (86219-3-RR, Clone: 250873D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86219-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86219-3-RR
Biolayer interferometry (BLl) kinetic assays of 86219-3-RR against Human HOXC10 were performed. The affinity constant is below 1 pM.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86219-3-PBS targets HOXC10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2655 Product name: Recombinant human HOXC10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-342 aa of BC001293 Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTGNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKTINLTDRQVKIWFQNRRMKLKKMNRENRIRELTSNFNFT Predict reactive species
Full Name	homeobox C10
Calculated Molecular Weight	342 aa, 38 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC001293
Gene Symbol	HOXC10
Gene ID (NCBI)	3226
RRID	AB_3744724
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NYD6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486).