HOXC4 Polyclonal antibody

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• KD/KO Validated

HOXC4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14321-1-AP

Synonyms

cp19, homeobox C4, Homeobox protein CP19, Homeobox protein Hox 3E, Homeobox protein Hox C4, HOX3, HOX3E, HOXC4

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Validation Data Gallery

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  WB analysis using 14321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14321-1-AP (HOXC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14321-1-AP

  WB result of HOXC4 antibody (14321-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HOXC4 transfected Jurkat cells.

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  IF Staining of HeLa using 14321-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14321-1-AP (HOXC4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-12 cells, U-251 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14321-1-AP targets HOXC4 in WB, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOXC4 fusion protein Ag5563
• Full Name: homeobox C4
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC050442
• Gene Symbol: HOXC4
• Gene ID (NCBI): 3221
• RRID: AB_2233196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The class I homeobox (HOX) family of homeodomain-containing transcription factors has 39 members in mammals. These genes are distributed throughout the genome in four clusters: HOXA (7p15), HOXB (17q21), HOXC (12q13), and HOXD (2q31). HOX genes are known to act as transcription factors, playing an essential regulatory role in the temporal and spatial development of the embryo. HOXC family members are not expressed in normal prostate tissue but increased expression of HOXC genes is commonly detected in prostate cancers. HOXC4 is expressed in GC B cells and HoxC4 expression is specifically up-regulated by the same stimuli that induce AID expression (LPS, CD154 and IL-4). The calculated molecular weight of HOXC4 is 30 kDa. Sometimes higher molecular weight around 39 kDa can also be observed, which may be a modified variant of HOXC4 (PMID: 23178148, 32012197, 35400027).

Publications

Species	Application	Title
human	WB	Oncotarget; Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression.; Authors - Chao Liu