HOXD13 Polyclonal antibody

• 

  WB analysis of HL-60 using 23520-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23520-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 23520-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 23520-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 23520-1-AP (HOXD13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HOXD13 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23520-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

---

Tested Applications

• Positive WB detected in: HL-60 cells, A549 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23520-1-AP targets HOXD13 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20093

  Product name: Recombinant human HOXD13 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-273 aa of BC148863

  Sequence: MSRAGSWDMDGLRADGGGAGGAPASSSSSSVAAAAASGQCRGFLSAPVFAGTHSGRAAAAAAAAAAAAAAASGFAYPGTSERTGSSSSSSSSAVVAARPEAPPAKECPAPTPAAAAAAPPSAPALGYGYHFGNGYYSCRMSHGVGLQQNALKSSPHASLGGFPVEKYMDVSGLASSSVPANEVPARAKEVSFYQGYTSPYQHVPGYIDMVSTFGSGEPRHEAYISMEGYQSWTLANGWNSQVYCTKDQPQGSHFWKSSFPGDVALNQPDMCVY

• Full Name: homeobox D13
• Calculated Molecular Weight: 343 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC148863
• Gene Symbol: HOXD13
• Gene ID (NCBI): 3239
• RRID: AB_2879291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35453
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.