Tested Applications
| Positive WB detected in | HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17004-1-AP targets HPD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10700 Product name: Recombinant human HPD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-393 aa of BC024287 Sequence: MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQAASFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM Predict reactive species |
| Full Name | 4-hydroxyphenylpyruvate dioxygenase |
| Calculated Molecular Weight | 393 aa, 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC024287 |
| Gene Symbol | HPD |
| Gene ID (NCBI) | 3242 |
| RRID | AB_2264310 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32754 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HPD antibody 17004-1-AP | Download protocol |
| IHC protocol for HPD antibody 17004-1-AP | Download protocol |
| IP protocol for HPD antibody 17004-1-AP | Download protocol |
| WB protocol for HPD antibody 17004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HPD Polyclonal antibody (Cat# 17004-1-AP) has 3 citations. These appear in Nephrol Dial Transplant, Brief Bioinform, and Hum Mol Genet. The associated research fields include diabetic kidney disease (ketone reabsorption in renal tubules), spatial transcriptomics of eyelid Moll glands, and tyrosine/homogentisic acid metabolism in alkaptonuria. Applications cited are WB and IF, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Nephrol Dial Transplant Key enzyme in charge of ketone reabsorption of renal tubular SMCT1 may be a new target in diabetic kidney disease | ||
Brief Bioinform Spotting a unicorn: spatial transcriptome analysis of the eyelid reveals gene regulatory networks enriched in Moll glands. | ||
Hum Mol Genet Specific knockout of kidney homogentisate 1,2-dioxygenase reveals that local metabolism of tyrosine and homogentisic acid is negligible in alkaptonuria. |
