HPD Polyclonal antibody

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  WB analysis of HepG2 using 17004-1-AP

  WB result of HPD antibody (17004-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPD transfected HepG2 cells.

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  WB analysis using 17004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17004-1-AP

  WB result of HPD antibody (17004-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HPD transfected HepG2 cells.

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  WB analysis using 17004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17004-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17004-1-AP (HPD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17004-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17004-1-AP (HPD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17004-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 17004-1-AP (HPD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17004-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GLOD3, EC:1.13.11.27, 4-hydroxyphenylpyruvic acid oxidase, 4-hydroxyphenylpyruvate dioxygenase, 4HPPD

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17004-1-AP targets HPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10700

  Product name: Recombinant human HPD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-393 aa of BC024287

  Sequence: MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQAASFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM

• Full Name: 4-hydroxyphenylpyruvate dioxygenase
• Calculated Molecular Weight: 393 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC024287
• Gene Symbol: HPD
• Gene ID (NCBI): 3242
• RRID: AB_2264310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32754
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nephrol Dial Transplant; Key enzyme in charge of ketone reabsorption of renal tubular SMCT1 may be a new target in diabetic kidney disease; Authors - Zhenhong Guo