HPD Monoclonal antibody

HPD Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3C9G2

Cat no : 67552-1-Ig

Synonyms

4 HPPD, 4HPPD, GLOD3, HPD, HPPDase, PPD

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Validation Data Gallery

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  WB analysis using 67552-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 67552-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67552-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67552-1-Ig (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67552-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67552-1-Ig (HPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67552-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67552-1-Ig (HPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, pig liver tissue, rat liver tissue, HepG2 cells, HEK-293 cells, HSC-T6 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67552-1-Ig targets HPD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human, zebrafish
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HPD fusion protein Ag10928
• Full Name: 4-hydroxyphenylpyruvate dioxygenase
• Calculated Molecular Weight: 393 aa, 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC024287
• Gene Symbol: HPD
• Gene ID (NCBI): 3242
• RRID: AB_2882766
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,zebrafish	WB	Pest Manag Sci; In vivo fluorescent screening for HPPD-targeted herbicide discovery; Authors - Yi-Xuan Fu