Tested Applications
| Positive WB detected in | HeLa cells, pig liver tissue, rat liver tissue, HepG2 cells, HEK-293 cells, HSC-T6 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67552-1-Ig targets HPD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10928 Product name: Recombinant human HPD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-393 aa of BC024287 Sequence: MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQAASFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM Predict reactive species |
| Full Name | 4-hydroxyphenylpyruvate dioxygenase |
| Calculated Molecular Weight | 393 aa, 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC024287 |
| Gene Symbol | HPD |
| Gene ID (NCBI) | 3242 |
| RRID | AB_2882766 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P32754 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HPD antibody 67552-1-Ig | Download protocol |
| WB protocol for HPD antibody 67552-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HPD Monoclonal antibody (3C9G2), catalog number 67552-1-Ig, has 6 total citations. These appear in Nature Communications, Cell Reports (×2), Journal of Agricultural and Food Chemistry, Life Sciences, and Pest Management Science. The research fields span hepatocellular carcinoma metabolism, colorectal cancer cuproptosis, RNA modification and oncogenic translation, proherbicide design targeting HPPD, papillary thyroid carcinoma cell cycle regulation, and fluorescent screening for HPPD-targeted herbicide discovery.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting tRNA-dependent tyrosine usage unveils a metabolic vulnerability in hepatocellular carcinoma. | ||
Cell Rep N6-methyladenosine in 28S rRNA promotes oncogenic mRNA translation and tyrosine catabolism | ||
Cell Rep HPD is a copper-binding protein that interacts with DLAT to promote colorectal cancer cuproptosis under copper stress | ||
J Agric Food Chem Design and Syntheses of Proherbicides Targeting 4-Hydroxyphenylpyruvate Dioxygenase | ||
Life Sci Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest | ||
