Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11035-1-AP targets HPGD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1499 Product name: Recombinant human HPGD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC018986 Sequence: MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALVDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGILDPPLIANGLITLIEDDALNGAIMKITTSKGIHFQDYDTTPFQAKTQ Predict reactive species |
| Full Name | hydroxyprostaglandin dehydrogenase 15-(NAD) |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC018986 |
| Gene Symbol | HPGD |
| Gene ID (NCBI) | 3248 |
| RRID | AB_2120407 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HPGD antibody 11035-1-AP | Download protocol |
| IHC protocol for HPGD antibody 11035-1-AP | Download protocol |
| IP protocol for HPGD antibody 11035-1-AP | Download protocol |
| WB protocol for HPGD antibody 11035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HPGD Polyclonal antibody (Cat# 11035-1-AP) has 1 citation. It appears in the International Journal of Molecular Sciences (2023), in a study investigating how 15-hydroxyprostaglandin dehydrogenase inhibits mesenchymal shifts in trophoblasts and decidual stromal cells in preeclampsia. The antibody was applied in WB, IHC, and IF on human samples. The associated research field is reproductive medicine and placental biology, specifically preeclampsia pathophysiology.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci The Enzyme 15-Hydroxyprostaglandin Dehydrogenase Inhibits a Shift to the Mesenchymal Pattern of Trophoblasts and Decidual Stromal Cells Accompanied by Prostaglandin Transporter in Preeclampsia |
