HPS1 Polyclonal antibody

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  WB analysis of L02 using 15077-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15077-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15077-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 15077-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15077-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15077-1-AP (HPS1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 15077-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HPS1 antibody (15077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 15077-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HPS1 antibody (15077-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

HPS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 15077-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, RIP, ELISA

Synonyms

HPS, Hermansky-Pudlak syndrome 1 protein, BLOC-3 complex member HPS1

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Tested Applications

• Positive WB detected in: Y79 cells, Transfected HEK-293 cells, Raji cells, L02 cells, K-562 cells, mouse lung tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

15077-1-AP targets HPS1 in WB, IF/ICC, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7149

  Product name: Recombinant human HPS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-324 aa of BC000175

  Sequence: MKCVLVATEGAEVLFYWTDQEFEESLRLKFGQSENEEEELPALEDQLSTLLAPVIISSMTMLEKLSDTYTCFSTENGNFLYVLHLFGECLFIAINGDHTESEGDLRRKLYVLKYLFEVHFGLVTVDGHLIRKELRPPDLAQRVQLWEHFQSLLWTYSRLREQEQCFAVEALERLIHPQLCELCIEALERHVIQAVNTSPERGGEEALHAFLLVHSKLLAFYSSHSASSLRPADLLALILLVQDLYPSESTAEDDIQPSPRRARSSQNIPVQQAWSPHSTGPTGGSSAETETDSFSLPEEYFTPAPSPGDQSSGEDRRKAGGNNS

• Full Name: Hermansky-Pudlak syndrome 1
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC000175
• Gene Symbol: HPS1
• Gene ID (NCBI): 3257
• RRID: AB_10694669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92902
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPS1, also named as Hermansky-Pudlak syndrome 1 protein, is a 700 amino acid protein, which is a component of multiple cytoplasmic organelles. HPS1 is Apparently crucial for their normal development and function and involved in intracellular protein sorting.HPS1 is involved in the biogenesis of early melanosomes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook ; KO Validated
		Am J Respir Cell Mol Biol; Dysregulation of Galectin-3: Implications for Hermansky-Pudlak Syndrome Pulmonary Fibrosis.; Authors - Cullinane Andrew R AR
human	RIP	Clin Exp Metastasis; Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1.; Authors - Tongxin Xu
human	WB	Mol Genet Metab; In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.; Authors - Yasuhiro Ikawa
human	WB	J Cell Biol; Rab32 family proteins regulate autophagosomal components recycling; Authors - Zhe Wu