HPS3 Polyclonal antibody

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• HPS3 Polyclonal antibody (10768-1-AP)

HPS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10768-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA
For FC, select conjugated version.

Synonyms

BLOC-2 complex member HPS3, Hermansky-Pudlak syndrome 3 protein

• 

  WB analysis of HeLa using 10768-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10768-1-AP (HPS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10768-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10768-1-AP (HPS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293T using 10768-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10768-1-AP (HPS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 10768-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10768-1-AP (HPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10768-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HPS3 antibody (10768-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10768-1-AP targets HPS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1209

  Product name: Recombinant human HPS3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 441-858 aa of BC022062

  Sequence: VLARTDWTVEDGLQKYERGLIFYINHSLYENLDEELNEELAAKVVQMFYVAEPKQVPHILCSPSMKNINPLTAMSYLRKLDTSGFSSILVTLTKAAVALKMGDLDMHRNEMKSHSEMKLVCGFILEPRLLIQQRKGQIVPTELALHLKETQPGLLVASVLGLQKNNKIGIEEADSFFKVLCAKDEDTIPQLLVDFWEAQLVACLPDVVLQELFFKLTSQYIWRLSKRQPPDTTPLRTSEDLINACSHYGLIYPWVHVVISSDSLADKNYTEDLSKLQSLICGPSFDIASIIPFLEPLSEDTIAGLSVHVLCRTRLKEYEQCIDILLERCPEAVIPYANHELKEENRTLWWKKLLPELCQRIKCGGEKYQLYLSSLKETLSIVAVELELKDFMNVLPEDGTATFFLPYLLYCSRKKPLT

• Full Name: Hermansky-Pudlak syndrome 3
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC022062
• Gene Symbol: HPS3
• Gene ID (NCBI): 84343
• RRID: AB_2120571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969F9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The HPS3 Polyclonal antibody (Cat# 10768-1-AP) has a total of 1 citation. It appears in PLoS One (2017), in a study investigating cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5. The research field is human genetics and lysosomal storage disorders. The antibody was used for Western blot (WB) on human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	PLoS One; Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.; Authors - Joshi Stephen