HPS6 Polyclonal antibody

• Featured Product
• KD/KO Validated

HPS6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11371-1-AP

Synonyms

Hermansky Pudlak syndrome 6, HPS6, RP11 302K17.1, Ru, Ruby eye protein homolog

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Validation Data Gallery

• 

  WB analysis of HeLa using 11371-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11371-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11371-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11371-1-AP

  IP result of anti-HPS6 (IP:11371-1-AP, 4ug; Detection:11371-1-AP 1:300) with HeLa cells lysate 3000ug.

• 

  IHC staining of human ovary tumor using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 11371-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

11371-1-AP targets HPS6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPS6 fusion protein Ag1929
• Full Name: Hermansky-Pudlak syndrome 6
• Calculated Molecular Weight: 775 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC011594
• Gene Symbol: HPS6
• Gene ID (NCBI): 79803
• RRID: AB_2120693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hermansky-Pudlak syndrome (HPS) defines a group of at least seven autosomal recessive disorders characterized by albinism and prolonged bleeding due to defects in the lysosome-related organelles, melanosomes and platelet-dense granules, respectively. HSP6, also named as Ru, regulates the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. It acts as cargo adapter for the dynein-dynactin motor complex to mediate the transport of lysosomes from the cell periphery to the perinuclear region.

Publications

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda ; KD Validated
human	WB	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya
mouse	WB	Am J Respir Cell Mol Biol; Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.; Authors - Seunghyi Kook
human	WB	Adv Sci (Weinh); Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase; Authors - Johannes Naß