Hemopexin Polyclonal antibody

Hemopexin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15736-1-AP

Synonyms

Beta 1B glycoprotein, hemopexin, HPX

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Validation Data Gallery

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  WB analysis of human plasma using 15736-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15736-1-AP (Hemopexin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse serum using 15736-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 15736-1-AP (Hemopexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human blood tissue, mouse serum, human plasma

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15736-1-AP targets Hemopexin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hemopexin fusion protein Ag8533
• Full Name: hemopexin
• Calculated Molecular Weight: 254 aa, 29 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC005395
• Gene Symbol: HPX
• Gene ID (NCBI): 3263
• RRID: AB_2878177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

Publications

Species	Application	Title
human	WB	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
rat	IHC	J Cell Biochem; Differential protein expression profiling by iTRAQ-2D-LC-MS/MS of rats treated with oxaliplatin.; Authors - Xianjun Sun
human	WB	Placenta; SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy; Authors - Yuxuan Jiang
mouse	IF	Elife; Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscle; Authors - Nurul Jannah Mohamed Nasir