HRC Polyclonal antibody

• 

  WB analysis of mouse heart using 18142-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 18142-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 18142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 18142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18142-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HRC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18142-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Sarcoplasmic reticulum histidine-rich calcium-binding protein

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, HeLa cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

18142-1-AP targets HRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12794

  Product name: Recombinant human HRC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 26-350 aa of BC069795

  Sequence: MTQQLRGDGLGFRNRNNSTGVAGLSEEASAELRHHLHSPRDHPDENKDVSTENGHHFWSHPDREKEDEDVSKEYGHLLPGHRSQDHKVGDEGVSGEEVFAEHGGQARGHRGHGSEDTEDSAEHRHHLPSHRSHSHQDEDEDEVVSSEHHHHILRHGHRGHDGEDDEGEEEEEEEEEEEEASTEYGHQAHRHRGHGSEEDEDVSDGHHHHGPSHRHQGHEEDDDDDDDDDDDDDDDDVSIEYRHQAHRHQGHGIEEDEDVSDGHHHRDPSHRHRSHEEDDNDDDDVSTEYGHQAHRHQDHRKEEVEAVSGEHHHHVPDHRHQGHRD

• Full Name: histidine rich calcium binding protein
• Calculated Molecular Weight: 699 aa, 80 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC069795
• Gene Symbol: HRC
• Gene ID (NCBI): 3270
• RRID: AB_10598013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23327
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
mouse	IF	Elife; Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy; Authors - Wei Li
mouse	WB	Chem Biol Interact; Vitamin D alleviates liver fibrosis by inhibiting histidine-rich calcium binding protein (HRC).; Authors - Wanyi Lu
human	WB,IHC	J Adv Res; Inhibition of lung cancer by vitamin D depends on downregulation of histidine-rich calcium-binding protein.; Authors - Ning Liu ; KO Validated