HRG Polyclonal antibody

HRG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26252-1-AP

Synonyms

histidine rich glycoprotein, HPRG, HRG, HRGP

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Validation Data Gallery

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  WB analysis of human plasma using 26252-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 26252-1-AP (HRG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 26252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26252-1-AP (HRG antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 26252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26252-1-AP (HRG antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26252-1-AP targets HRG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HRG fusion protein Ag16278
• Full Name: histidine-rich glycoprotein
• Calculated Molecular Weight: 525 aa, 60 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC069574
• Gene Symbol: HRG
• Gene ID (NCBI): 3273
• RRID: AB_2880447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Mol Med; Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation; Authors - Wei Jiang
rat		Front Pharmacol; 17β-Estradiol Enhances Schwann Cell Differentiation via the ERβ-ERK1/2 Signaling Pathway and Promotes Remyelination in Injured Sciatic Nerves.; Authors - Yun Gu