HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody

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  Various lysates were subjected to SDS-PAGE followed by western blot with Actin mouse monoclonal antibody (60008-1-Ig, IgM). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. Note that a higher signal is obtained using RGAM001 as it recognizes multiple epitopes.

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  Various lysates were subjected to SDS-PAGE, followed by western blot with Beta-Tubulin mouse monoclonal antibody (66240-1-Ig, IgG2a). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2a.

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  Various lysates were subjected to SDS-PAGE followed by western blot with FAK mouse monoclonal antibody (66258-1-Ig, IgG2c). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2c.

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  Various lysates were subjected to SDS-PAGE followed by western blot with Gelsolin mouse monoclonal antibody (66280-1-Ig, IgA). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgA.

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  Various lysates were subjected to SDS-PAGE, followed by western blot with NRF1 mouse monoclonal antibody (66832-1-Ig, IgG2b). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG2b.

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  Various lysates were subjected to SDS-PAGE followed by western blot with EIF3E mouse monoclonal antibody (67095-1-Ig, IgG1). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG1.

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  Various lysates were subjected to SDS-PAGE followed by western blot with ZNF174 mouse monoclonal antibody (68426-1-Ig, IgG3). HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701) and Multi-rAb HRP-Goat Anti-Mouse IgG (H+L) Recombinant Secondary Antibody (RGAM001) were used at 1: 20000 for detection. The result shows that RGAM701 does not react with mouse IgG3.

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  Direct ELISA was performed by coating mouse monoclonal antibodies with different isotypes followed by signal development using HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody (RGAM701). The result indicates that RGAM701 strongly binds to Mouse IgM and does not react with Mouse IgGs or mouse IgA.

HRP-Goat Anti-Mouse IgM-Specific Recombinant Secondary Antibody is a precisely engineered recombinant monoclonal antibody.

Cat No. RGAM701

Applications

ELISA, WB, Dot blot

Conjugate

HRP

Host

Goat

Reactivity

Mouse

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Product Information

RGAM701 targets Mouse IgM in ELISA and Western blot. It can bind to Mouse IgM and does not react with Mouse IgG or IgA.

• Concentration: 1.0 mg/mL
• Applications: ELISA, WB, Dot blot
• Host Species: Goat
• Species Reactivity: Mouse
• Physical State: Liquid
• Suggested Dilution Range: 1:10,000-1:100,000 for ELISA; 1:10,000-1:200,000 for western blotting with ECL substrates (1:10,000-1:40,000 is suggested for most systems).
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Storage Buffer: PBS with 50% glycerol, 10 mg/mL BSA, 0.1% Proclin-300, pH 7.4.
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Clonality: Recombinant monoclonal
• RRID: AB_3668657
• Note: This product is for research use only, not for diagnostic or therapeutic use.

Background

This secondary antibody reacts with Mouse IgM but does not react with Mouse IgG or IgA.