Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. SA00001-1 (HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) as secondary antibody.

Cat No. SA00001-1

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Concentration	0.2 mg/mL
Applications	ELISA, WB, Dot blot
Host Species	Goat
Species Reactivity	Mouse
Physical State	Liquid
Suggested Dilution Range	1:2000-1:10,000 for western blotting with ECL substrates 1:1000-1:20,000 for ELISA and Western blotting with chromogenic substrates
Purity	The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Storage Buffer	0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 3 mg/ml BSA, pH 7.6.
Storage Instructions	Store at -20°C. Stable for one year after shipment.
Stabilizer	3 mg/ml BSA
Clonality	Polyclonal
RRID	AB_2722565

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH aidan (Verified Customer) (07-30-2024)	Worked very well, great price. Would highly recommend Applications: Western Blot Primary Antibody Dilution: 1:4000 Cell Tissue Type: Mouse retinal
FH Paula (Verified Customer) (07-26-2024)	Works well for Western Blotting (e.g., ATP synthase V) Applications: Western Blot Primary Antibody Dilution: 1:4000
FH Brogan (Verified Customer) (11-09-2022)	Worked against fluorophore antibody from abcam. Very efficeint and cheap in comparison to the other companies. Would buy again. Applications: Western Blot, Immunofluorescence, Cell culture, Other
FH David (Verified Customer) (08-07-2019)	Protein in yeast expression system. We ran a chemi blot using this secondary. Applications: Western Blot, Primary Antibody Dilution: 1/10000 Cell Tissue Type: Yeast

HRP-conjugated Goat Anti-Mouse IgG(H+L)