HRSP12 Polyclonal antibody

HRSP12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12930-1-AP

Synonyms

heat responsive protein 12, HRSP12, P14.5, PSP, Ribonuclease UK114, UK114, UK114 antigen homolog

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Validation Data Gallery

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  WB analysis of human liver using 12930-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12930-1-AP (HRSP12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12930-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12930-1-AP (HRSP12 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 12930-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12930-1-AP (HRSP12 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12930-1-AP targets HRSP12 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HRSP12 fusion protein Ag3628
• Full Name: heat-responsive protein 12
• Calculated Molecular Weight: 14.5 kDa
• Observed Molecular Weight: 14.5 kDa
• GenBank Accession Number: BC010280
• Gene Symbol: HRSP12
• Gene ID (NCBI): 10247
• RRID: AB_2119169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonuclease UK114, also named HRSP12, is found predominantly in human adult kidney and liver and is responsible for inhibiting translation by cleaving mRNA. UK114 is mostly expressed in cytoplasm, but occasionally in nucleus in less differentiated cells. UK114 cleaves phosphodiester bonds only in single-stranded RNA. The levels of both the mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues, suggesting that ribonuclease UK114 may be an important biomarker for heptatic carcinoma. Current antibody catalog# 12930-1-AP is a rabbit polyclonal antibody raised from human full-length UK114.

Publications

Species	Application	Title
human, rat	WB	Mol Cell Biol; Y-box binding protein 1 and RNase UK114 mediate monocyte chemoattractant protein 1 mRNA stability in vascular smooth muscle cells.; Authors - Dhawan Latika L
human	WB,IHC	Genes Genomics; P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma; Authors - Sa Song