HS1BP3 Polyclonal antibody

HS1BP3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12845-1-AP

Synonyms

ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3

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Validation Data Gallery

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  WB analysis of HT-1080 using 12845-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12845-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12845-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12845-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 12845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 12845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 12845-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12845-1-AP targets HS1BP3 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HS1BP3 fusion protein Ag3880
• Full Name: HCLS1 binding protein 3
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC027947
• Gene Symbol: HS1BP3
• Gene ID (NCBI): 64342
• RRID: AB_10642005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.