HSBP1 Polyclonal antibody

HSBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10169-2-AP

Synonyms

HSBP1, HSF1BP

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Validation Data Gallery

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  WB analysis of mouse brain using 10169-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10169-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10169-2-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10169-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10169-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10169-2-AP (HSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10169-2-AP

  IP result of anti-HSBP1 (IP:10169-2-AP, 4ug; Detection:10169-2-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10169-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10169-2-AP (HSBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 10169-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10169-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10169-2-AP (HSBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, HT-1080 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse liver tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10169-2-AP targets HSBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSBP1 fusion protein Ag0225
• Full Name: heat shock factor binding protein 1
• Calculated Molecular Weight: 8.5 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC007515
• Gene Symbol: HSBP1
• Gene ID (NCBI): 3281
• RRID: AB_2119501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Endocrinol; Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia; Authors - Li-Li Chen