HSD17B1 Polyclonal antibody

HSD17B1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 25334-1-AP

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

20 alpha-hydroxysteroid dehydrogenase, 20 alpha HSD, 17β-HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of human placenta using 25334-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 25334-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of human placenta using 25334-1-AP
IP result of anti-HSD17B1 (IP:25334-1-AP, 4ug; Detection:25334-1-AP 1:500) with human placenta tissue lysate 2600 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human endometrial cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human endometrial cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human placenta tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human placenta tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25334-1-AP targets HSD17B1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17886 Product name: Recombinant human HSD17B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC104752 Sequence: MARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVTEGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKFALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRSAVGDPELGDPPAAPQ Predict reactive species
Full Name	hydroxysteroid (17-beta) dehydrogenase 1
Calculated Molecular Weight	328 aa, 35 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC104752
Gene Symbol	HSD17B1
Gene ID (NCBI)	3292
RRID	AB_2880032
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14061
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HSD17B1 antibody 25334-1-AP	Download protocol
IP protocol for HSD17B1 antibody 25334-1-AP	Download protocol
WB protocol for HSD17B1 antibody 25334-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HSD17B1 Polyclonal antibody (Cat# 25334-1-AP) has 11 total citations. These appear in journals including Gut Microbes, J Nanobiotechnology, Science of the Total Environment, Journal of Translational Medicine, Human Reproduction Open, Environmental Research, Cells, Aquaculture Nutrition, Reproductive Toxicology, Journal of Steroid Biochemistry and Molecular Biology, and Reproductive Sciences. The research fields span reproductive endocrinology (estrogen synthesis, ovarian function, granulosa cell biology), environmental toxicology (microplastics, PFOS/PFOA, cadmium exposure), autophagy, and mitochondrial homeostasis.

Species	Application	Title
mouse	IHC	Gut Microbes High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis Authors - Jingyi Wen View Article
mouse	WB	J Nanobiotechnology Ti3C2 nanosheet-induced autophagy derails ovarian functions Authors - Limei Yang View Article
mouse	WB	Sci Total Environ Polystyrene microplastics trigger testosterone decline via GPX1 Authors - Jiayuan Qu View Article
human	WB	J Transl Med An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer Authors - Guan-Yu Ruan View Article
human	IHC	Hum Reprod Open Progressively diminished estrogen signaling concordant with increased fibrosis in ectopic endometrium Authors - Jichan Nie View Article
human	WB	Environ Res Exposure-effect of PFOS and PFOA on lung function: An integrated approach with epidemiological, cellular, and animal studies Authors - Jia-Xin Zhou View Article