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WB analysis of human placenta using 25334-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 25334-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 25334-1-AP
IP result of anti-HSD17B1 (IP:25334-1-AP, 4ug; Detection:25334-1-AP 1:500) with human placenta tissue lysate 2600 ug.
IHC staining of human placenta using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 25334-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens).

HSD17B1 Polyclonal antibody

Name: HSD17B1 Polyclonal antibody
Brand: Proteintech
SKU: 25334-1-AP

HSD17B1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 25334-1-AP

Publications(7)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

20 alpha-hydroxysteroid dehydrogenase, 20 alpha HSD, 17β-HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human placenta tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human placenta tissue, human colon cancer tissue, human endometrial cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 1 publications below

Product Information

25334-1-AP targets HSD17B1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17886 Product name: Recombinant human HSD17B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC104752 Sequence: MARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVTEGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKFALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRSAVGDPELGDPPAAPQ Predict reactive species
Full Name	hydroxysteroid (17-beta) dehydrogenase 1
Calculated Molecular Weight	328 aa, 35 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC104752
Gene Symbol	HSD17B1
Gene ID (NCBI)	3292
RRID	AB_2880032
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14061
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HSD17B1 antibody 25334-1-AP	Download protocol
IP protocol for HSD17B1 antibody 25334-1-AP	Download protocol
WB protocol for HSD17B1 antibody 25334-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Transl Med An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer Authors - Guan-Yu Ruan View Article
mouse	WB	Cells HDAC1-Mediated lncRNA Stimulatory Factor of Follicular Development to Inhibit the Apoptosis of Granulosa Cells and Regulate Sexual Maturity through miR-202-3p-COX1 Axis Authors - Xiaofeng Zhou View Article
mouse	WB	J Nanobiotechnology Ti3C2 nanosheet-induced autophagy derails ovarian functions Authors - Limei Yang View Article
mouse	WB	Sci Total Environ Polystyrene microplastics trigger testosterone decline via GPX1 Authors - Jiayuan Qu View Article
mouse	IHC	Gut Microbes High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis Authors - Jingyi Wen View Article
	WB	Aquac Nutr Dietary DHA Regulated the Androgen Production in Male Chinese Tongue Sole Cynoglossus semilaevis Authors - Jiahao Liu View Article