HSD17B1 Polyclonal antibody

HSD17B1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27830-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 1, 20 alpha HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, HSD17, HSD17B1, SDR28C1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis of human placenta using 27830-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 27830-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 27830-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 27830-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 27830-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 27830-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 27830-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for HSD17B1 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human placenta tissue
Positive IHC detected in	human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27830-1-AP targets HSD17B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27213 Product name: Recombinant human HSD17B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 191-328 aa of BC104752 Sequence: TAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRSAVGDPELGDPPAAPQ Predict reactive species
Full Name	hydroxysteroid (17-beta) dehydrogenase 1
Calculated Molecular Weight	328 aa, 35 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC104752
Gene Symbol	HSD17B1
Gene ID (NCBI)	3292
RRID	AB_2880985
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14061
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for HSD17B1 antibody 27830-1-AP	Download protocol
WB protocol for HSD17B1 antibody 27830-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The HSD17B1 Polyclonal antibody (Cat# 27830-1-AP) has 1 citation. It appears in Reproductive Sciences (Impact Factor 2.8), in a study on Klotho deficiency driving ovarian aging through granulosa cell senescence and dysfunction. The research field is reproductive biology, specifically ovarian aging and granulosa cell biology. The antibody was used in Western Blot (WB) with human samples.