HSD17B11 Polyclonal antibody

HSD17B11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17301-1-AP

Synonyms

17 beta HSD 11, 17 beta HSD XI, 17 BETA HSD11, 17 BETA HSDXI, 17betaHSD11, 17betaHSDXI, 17bHSD11, DHRS8, HSD17B11, PAN1B, RETSDR2, SDR16C2, UNQ207/PRO233

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Validation Data Gallery

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  WB analysis using 17301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17301-1-AP (HSD17B11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas，
• Positive IHC detected in: human kidney tissue, human brain tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17301-1-AP targets HSD17B11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B11 fusion protein Ag11182
• Full Name: hydroxysteroid (17-beta) dehydrogenase 11
• Calculated Molecular Weight: 300 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC016367
• Gene Symbol: HSD17B11
• Gene ID (NCBI): 51170
• RRID: AB_2119810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Syst Biol Reprod Med; Identification of ferroptotic genes and phenotypes in idiopathic nonobstructive azoospermia; Authors - Chen Liao