HSD17B11 Polyclonal antibody

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  WB analysis using 17301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17301-1-AP (HSD17B11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

HSD17B11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17301-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 11, 17 beta HSD XI, 17 BETA HSD11, 17 BETA HSDXI, 17betaHSD11, 17betaHSDXI, 17bHSD11, DHRS8, HSD17B11, PAN1B, RETSDR2, SDR16C2, UNQ207/PRO233

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas，
• Positive IHC detected in: human kidney tissue, human brain tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

17301-1-AP targets HSD17B11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B11 fusion protein Ag11182
• Full Name: hydroxysteroid (17-beta) dehydrogenase 11
• Calculated Molecular Weight: 300 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC016367
• Gene Symbol: HSD17B11
• Gene ID (NCBI): 51170
• RRID: AB_2119810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NBQ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Syst Biol Reprod Med; Identification of ferroptotic genes and phenotypes in idiopathic nonobstructive azoospermia; Authors - Chen Liao