Tested Applications
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13415-1-AP targets HSD17B3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4081 Product name: Recombinant human HSD17B3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-177 aa of BC034281 Sequence: VRFSRCVLLNYWKVLPKSFLRSMGQWAVITGAGDGIGKAYSFELAKRGLNVVLISRTLEKLEAIATEIERTTGRSVKIIQADFTKDDIYEHIKEKLAGLEIGILVNNVGMLPNLLPSHFLNAPDEIQSLIHCNITSVVKMTQLILKHMESRQK Predict reactive species |
| Full Name | hydroxysteroid (17-beta) dehydrogenase 3 |
| Calculated Molecular Weight | 310 aa, 35 kDa |
| GenBank Accession Number | BC034281 |
| Gene Symbol | HSD17B3 |
| Gene ID (NCBI) | 3293 |
| RRID | AB_2877946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37058 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSD17B3 antibody 13415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HSD17B3 polyclonal antibody (Cat# 13415-1-AP) has 19 citations published in journals including Nature Communications, European Journal of Endocrinology, FASEB Journal, Journal of Steroid Biochemistry and Molecular Biology, Frontiers in Cell and Developmental Biology, Andrology, and others. Research fields span steroid biosynthesis, Leydig cell biology, testicular development, androgen signaling, reproductive endocrinology, environmental toxicology effects on steroidogenesis, and tissue engineering.
| Species | Application | Title |
|---|---|---|
Environ Pollut Multi-omics analysis reveals fluorinated liquid crystal monomers BDPrB/EDPrB exposure disrupts the steroid biosynthesis function via inhibition of CYP17A1. | ||
J Ethnopharmacol Tianjing Zelan formula improves polycystic ovary syndrome by regulating steroid biosynthesis and arachidonic acid metabolism. | ||
Eur J Endocrinol Persistence of foetal testicular features in patients with defective androgen signalling | ||
Eur J Endocrinol Transcriptional comparison of testicular adrenal rest tumors with fetal and adult tissues | ||
Ecotoxicol Environ Saf Integrating network pharmacology and experimental validation to decipher the protective effect of icariin against testicular aging. |
