HSD17B6 Polyclonal antibody

HSD17B6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11855-1-AP

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

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Validation Data Gallery

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  WB analysis using 11855-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11855-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 11855-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, rat liver tissue
• Positive IHC detected in: human lung cancer tissue, human prostate hyperplasia tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11855-1-AP targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B6 fusion protein Ag2427
• Full Name: hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
• Calculated Molecular Weight: 317 aa, 36 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC020710
• Gene Symbol: HSD17B6
• Gene ID (NCBI): 8630
• RRID: AB_2295487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Breast Cancer Res Treat; The presence and impact of estrogen metabolism on the biology of triple-negative breast cancer.; Authors - Keely May McNamara
human	WB	Cancer Cell Int; Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma.; Authors - Lei Lv
human	WB	Cell Death Discov; HSD17B6 downregulation predicts poor prognosis and drives tumor progression via activating Akt signaling pathway in lung adenocarcinoma; Authors - Tian Tian