HSD17B7 Polyclonal antibody

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  WB analysis using 14854-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14854-1-AP

  WB result of HSD17B7 antibody (14854-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 14854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14854-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14854-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14854-1-AP (HSD17B7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14854-1-AP

  IP result of anti-HSD17B7 (IP:14854-1-AP, 4ug; Detection:14854-1-AP 1:1000) with MCF-7 cells lysate 3200ug.

• Featured Product
• KD/KO Validated

HSD17B7 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14854-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

17 beta HSD 7, 3 keto steroid reductase, HSD17B7, PRAP, SDR37C1

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, Jurkat cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14854-1-AP targets HSD17B7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6639

  Product name: Recombinant human HSD17B7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-229 aa of BC065246

  Sequence: MRKVVLITGASSGIGLALCKRLLAEDDELHLCLACRNMSKAEAVCAALLASHPTAEVTIVQVDVSNLQSVFRASKELKQRFQRLDCIYLNAGIMPNPQLNIKALFFGLFSRKVIHMFSTAEGLLTQGDKITADGLQEVFETNVFGHFILIRELEPLLCHSDNPSQLIWTSSRSARKSNFSLEDFQHSKGKEPYSSSKYATDLLSVALNRNFNQQGLYSNVACPGTALTN

• Full Name: hydroxysteroid (17-beta) dehydrogenase 7
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 32-38 kDa
• GenBank Accession Number: BC065246
• Gene Symbol: HSD17B7
• Gene ID (NCBI): 51478
• RRID: AB_10951483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56937
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	Reprod Biol Endocrinol; The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.; Authors - Xiaoyu Tu
mouse	IF	Cell Rep; Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.; Authors - Taha A Jan
human	WB	Nutrients; Malvidin-3-O-Glucoside Ameliorates Cadmium-Mediated Cell Dysfunction in the Estradiol Generation of Human Granulosa Cells; Authors - Shuer Liang
mouse	WB	Mol Hum Reprod; Semaphorin 4C regulates ovarian steroidogenesis through RHOA/ROCK1-mediated actin cytoskeleton rearrangement; Authors - Dan Chen
mouse	WB	Mol Cell Endocrinol; High concentrations of progesterone inhibit the expression of genes related to steroid metabolism in MA-10 Leydig cells; Authors - Liel-Sarah Izichkis