HSD17B7 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSD17B7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16925-1-AP

Synonyms

Hsd17b_2, HSD17B7, HSD17B7P2

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Validation Data Gallery

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  WB analysis of HEK-293 using 16925-1-AP

  WB result of HSD17B7 antibody (16925-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

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  WB analysis of human liver using 16925-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16925-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16925-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 16925-1-AP

  IP result of anti-HSD17B7 (IP:16925-1-AP, 4ug; Detection:16925-1-AP 1:500) with L02 cells lysate 2800ug.

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  IHC staining of human liver using 16925-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16925-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human liver tissue, L02 cells, HEK-293 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16925-1-AP targets HSD17B7 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B7 fusion protein Ag10315
• Full Name: hydroxysteroid (17-beta) dehydrogenase 7 pseudogene 2
• Calculated Molecular Weight: 26 kDa, 37 kDa
• Observed Molecular Weight: 32-37 kDa
• GenBank Accession Number: BC071987
• Gene Symbol: HSD17B7
• Gene ID (NCBI): 158160
• RRID: AB_2119975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.