HSDL1 Polyclonal antibody

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  WB analysis using 16988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 16988-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 16988-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16988-1-AP (HSDL1 antibody) at dilution of 1:100 (under 40x lens).

HSDL1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16988-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HSDL1, SDR12C3

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Tested Applications

• Positive WB detected in: HEK-293 cells, fetal human brain tissue
• Positive IHC detected in: human breast cancer tissue, human kidney tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16988-1-AP targets HSDL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSDL1 fusion protein Ag10588
• Full Name: hydroxysteroid dehydrogenase like 1
• Calculated Molecular Weight: 330 aa, 37 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC018084
• Gene Symbol: HSDL1
• Gene ID (NCBI): 83693
• RRID: AB_2120235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3SXM5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy; Authors - Zhao-Xin Zhang
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico