HSDL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSDL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15631-1-AP

Synonyms

Short chain dehydrogenase/reductase family 13C member 1, SDR13C1, Hydroxysteroid dehydrogenase-like protein 2, EC:1.-.-.-, C9orf99

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Validation Data Gallery

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  WB analysis using 15631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15631-1-AP (HSDL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15631-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (15631-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Product Information

15631-1-AP targets HSDL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSDL2 fusion protein Ag7987
• Full Name: hydroxysteroid dehydrogenase like 2
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC004331
• Gene Symbol: HSDL2
• Gene ID (NCBI): 84263
• RRID: AB_2120238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

Publications

Species	Application	Title
human	WB	Proteomics; Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.; Authors - Songlin Zeng
human	IF	Cancer Manag Res; Ectopic expression of HSDL2 is related to cell proliferation and prognosis in breast cancer.; Authors - Bing Dong
human	IHC,WB	Onco Targets Ther; HSDL2 Acts as a Promoter in Pancreatic Cancer by Regulating Cell Proliferation and Lipid Metabolism.; Authors - Anna Han ; KD Validated
human	WB,FC	Chin Med; Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression.; Authors - Wen-Bei Liu ; KD Validated
human	IHC	Nan Fang Yi Ke Da Xue Xue Bao; [HSDL2 overexpression promotes rectal cancer progression by regulating cancer cell cycle and promoting cell proliferation]; Authors - Y Cheng ; KD Validated
mouse,human	WB,IF	Sci Adv; HSDL2 links nutritional cues to bile acid and cholesterol homeostasis; Authors - Nolwenn Samson