HSF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10706-1-AP

Synonyms

Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2

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Validation Data Gallery

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  WB analysis using 10706-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10706-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 10706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10706-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10706-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, A549 cells
• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

10706-1-AP targets HSF2 in WB, IHC, ELISA applications and shows reactivity with human, Mouse, rat samples.

• Tested Reactivity: human, Mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSF2 fusion protein Ag1067
• Full Name: heat shock transcription factor 2
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC005329
• Gene Symbol: HSF2
• Gene ID (NCBI): 3298
• RRID: AB_2120443
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Am J Cancer Res; HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.; Authors - Li-Na Yang ; KD Validated