Tested Applications
| Positive WB detected in | human testis tissue, mouse adipose tissue, rat adipose tissue |
| Positive IHC detected in | mouse testis tissue, mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brown adipose tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84540-1-RR targets HSL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag11360 Product name: Recombinant human LIPE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 728-1076 aa of BC070041 Sequence: GNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH Predict reactive species |
| Full Name | lipase, hormone-sensitive |
| Calculated Molecular Weight | 117 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC070041 |
| Gene Symbol | HSL |
| Gene ID (NCBI) | 3991 |
| RRID | AB_3672045 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q05469 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HSL antibody 84540-1-RR | Download protocol |
| IHC protocol for HSL antibody 84540-1-RR | Download protocol |
| WB protocol for HSL antibody 84540-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HSL Recombinant monoclonal antibody (241875B11), catalog number 84540-1-RR, has 1 total citation. It appears in the journal Food Chemistry and Toxicology. The associated research field involves integrative multi-omics analysis identifying FAAH and LIPE as candidate targets in OBS-induced spermatogenic and fertility impairment.
| Species | Application | Title |
|---|---|---|
Food Chem Toxicol Integrative multi-omics analysis reveals FAAH and LIPE as candidate targets in OBS-induced spermatogenic and fertility impairment. |
