HSP20 Monoclonal antibody

HSP20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1G7D2

Cat no : 67327-1-Ig

Synonyms

HspB6, Heat shock protein family B member 6, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock 20 kDa-like protein p20

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Validation Data Gallery

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  WB analysis using 67327-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67327-1-Ig (HSP20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 67327-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67327-1-Ig (HSP20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 67327-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of U-118 MG using 67327-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U-118 MG cells using HSP20 antibody (67327-1-Ig, Clone: 1G7D2 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: pig skeletal muscle tissue, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: U-118 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:850-1:3400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

67327-1-Ig targets HSP20 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP20 fusion protein Ag10484
• Full Name: heat shock protein, alpha-crystallin-related, B6
• Calculated Molecular Weight: 160 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC068046
• Gene Symbol: HSP20
• Gene ID (NCBI): 126393
• RRID: AB_2882586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.

Publications

Species	Application	Title
human	IHC	Lab Invest; HSPB6 deficiency promotes the development of aortic dissection and rupture; Authors - Shiqi Gao