Multi-rAb® HSP27 Multi-Recombinant antibody

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• Multi-rAb® HSP27 Multi-Recombinant antibody (RMX00089)

HSP27 Multi-Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. RMX00089

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HSPB1, CMT2F, Estrogen-regulated 24 kDa protein, HMN2B, Hsp25

• 

  WB analysis using RMX00089

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00089 (HSP27 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using RMX00089

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HSP27 antibody (RMX00089) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00089 targets HSP27 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  FusionProtein

• Full Name: heat shock 27kDa protein 1
• Calculated Molecular Weight: 23kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: NM_001540.5
• Gene Symbol: HSPB1
• Gene ID (NCBI): 3315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P04792
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.