HSP47/SERPINH1 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 83689-4-RR (same clone as 83689-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83689-4-RR (HSP47/SERPINH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

  WB result of HSP47/SERPINH1 antibody (83689-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47/SERPINH1 transfected HeLa cells. This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00677-2

  HepG2, Hela and MCF-7 cells were cultured in DMEM supplemented with 10% fetal bonive serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Aliquots of the cell culture supernates were removed. Cells were lysed and assayed for human HSP47/SERPINH1. Human HSP47/SERPINH1 in HepG2 cell lysate measured 17.9 ng/mL. Human HSP47/SERPINH1 in Hela cell lysate measured 56.9 ng/mL. Human HSP47/SERPINH1 in MCF-7 cell lysate measured 35.1 ng/mL.

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  Sandwich ELISA standard curve of MP00677-2

  Sandwich ELISA standard curve of MP00677-2, Human HSP47/SERPINH1 Recombinant Matched Antibody Pair - PBS only. 83689-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1201. 83689-3-PBS was HRP conjugated as the detection antibody. Range: 0.23-15 ng/mL

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  IHC staining of human ovarian cancer using 83689-4-RR (same clone as 83689-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83689-4-RR (HSP47/SERPINH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47/SERPINH1 antibody (83689-4-RR, Clone: 240752F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83689-4-RR (same clone as 83689-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47/SERPINH1 antibody (83689-4-RR, Clone: 240752F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83689-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83689-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83689-4-RR against Human HSP47/SERPINH1 were performed. The affinity constant is 5.99 nM.

• Featured Product
• KD/KO Validated

HSP47/SERPINH1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 83689-4-PBS

Clone No.240752F2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

HSP47, SERPINH1, 240752F2, 47 kDa heat shock protein, Arsenic-transactivated protein 3

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Product Information

83689-4-PBS targets HSP47/SERPINH1 as part of a matched antibody pair:

MP00677-2: 83689-4-PBS capture and 83689-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1201

  Product name: Recombinant Human HSP47/SERPINH1 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 19-418 aa of NM_001235.5

  Sequence: AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

• Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Calculated Molecular Weight: 46kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_001235.5
• Gene Symbol: HSP47/SERPINH1
• Gene ID (NCBI): 871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P50454
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.