HSP70 Recombinant antibody

HSP70 Recombinant Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, yeast

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4E10

Cat no : 80735-1-RR

Synonyms

FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, C6 cells, the whole yeast
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

80735-1-RR targets HSP70 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, yeast samples.

Tested Reactivity Human, mouse, rat, yeast
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSP70 fusion protein Ag1446
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC009322
Gene symbol HSPA1A
Gene ID (NCBI) 3303
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker

Protocols

Product Specific Protocols
WB protocol for HSP70 antibody 80735-1-RRDownload protocol
IHC protocol for HSP70 antibody 80735-1-RRDownload protocol
IF protocol for HSP70 antibody 80735-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols