HSP90 Monoclonal antibody, PBS Only

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  WB analysis using 67450-1-Ig (same clone as 67450-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67450-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67450-1-Ig (same clone as 67450-1-PBS)

  WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67450-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HSP90 Monoclonal Antibody for WB, ELISA

Cat No. 67450-1-PBS

Clone No.2D3D1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HSP90AB1, 2D3D1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

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Product Information

67450-1-PBS targets HSP90 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28379

  Product name: Recombinant human HSP90AB1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 330-710 aa of BC014485

  Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP

• Full Name: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Calculated Molecular Weight: 723 aa, 83 kDa
• Observed Molecular Weight: 83-90 kDa
• GenBank Accession Number: BC014485
• Gene Symbol: HSP90AB1
• Gene ID (NCBI): 3326
• RRID: AB_2882684
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08238
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.