HSPA12B Polyclonal antibody

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  WB analysis using 19016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19016-1-AP (HSPA12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19016-1-AP

  IP result of anti-HSPA12B (IP:19016-1-AP, 4ug; Detection:19016-1-AP 1:800) with mouse brain tissue lysate 1840 ug.

HSPA12B Polyclonal Antibody for WB, IP, ELISA

Cat No. 19016-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Heat shock protein family A member 12B, heat shock 70kD protein 12B, Heat shock 70 kDa protein 12B, C20orf60

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, mouse lung tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19016-1-AP targets HSPA12B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13544

  Product name: Recombinant human HSPA12B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 341-686 aa of BC110881

  Sequence: KELYKASGGPYGAVGVDLAFEQLLCRIFGEDFIATFKRQRPAAWVDLTIAFEARKRTAGPHRAGALNISLPFSFIDFYRKQRGHNVETALRRSSVNFVKWSSQGMLRMSCEAMNELFQPTVSGIIQHIEALLARPEVQGVKLLFLVGGFAESAVLQHAVQAALGARGLRVVVPHDVGLTILKGAVLFGQAPGVVRVRRSPLTYGVGVLNRFVPGRHPPEKLLVRDGRRWCTDVFERFVAAEQSVALGEEVRRSYCPARPGQRRVLINLYCCAAEDARFITDPGVRKCGALSLELEPADCGQDTAGAPPGRREIRAAMQFGDTEIKVTAVDVSTNRSVRASIDFLSN

• Full Name: heat shock 70kD protein 12B
• Calculated Molecular Weight: 686 aa, 76 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC110881
• Gene Symbol: HSPA12B
• Gene ID (NCBI): 116835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96MM6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.