HSPA1L Monoclonal antibody

HSPA1L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3E7H2

Cat no : 66780-1-Ig

Synonyms

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

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Validation Data Gallery

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  WB analysis of HeLa using 66780-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66780-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66780-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66780-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66780-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66780-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 66780-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66780-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66780-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66780-1-Ig (HSPA1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66780-1-Ig targets HSPA1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPA1L fusion protein Ag5182
• Full Name: heat shock 70kDa protein 1-like
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC034483
• Gene Symbol: HSPA1L
• Gene ID (NCBI): 3305
• RRID: AB_2882125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)

Publications

Species	Application	Title
mouse	IF	J Cachexia Sarcopenia Muscle; The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol; Authors - Wenqian Zhao