HSPA2 Monoclonal antibody

HSPA2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A4A9

Cat no : 66291-1-Ig

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2



Tested Applications

Positive WB detected inHepG2 cells, ROS1728 cells, HeLa cells
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

66291-1-Ig targets HSPA2 in WB, IHC,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSPA2 fusion protein Ag24228
Full Name heat shock 70kDa protein 2
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC036107
Gene symbol HSPA2
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Protocols

Product Specific Protocols
WB protocol for HSPA2 antibody 66291-1-IgDownload protocol
IHC protocol for HSPA2 antibody 66291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

Protein Quality Control Activation and Microtubule Remodeling in Hypertrophic Cardiomyopathy.

Authors - Larissa M Dorsch
humanWB

Front Cardiovasc Med

Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy.

Authors - Maike Schuldt
humanWB

Clin Epigenetics

Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations.

Authors - J Pei