HSPA2 Monoclonal antibody

HSPA2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, rat, mouse







Cat no : 66291-1-Ig


Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

Tested Applications

Positive WB detected inHepG2 cells, ROS1728 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66291-1-Ig targets HSPA2 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSPA2 fusion protein Ag24228
Full Name heat shock 70kDa protein 2
Calculated Molecular Weight 639 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC036107
Gene Symbol HSPA2
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)


Product Specific Protocols
WB protocol for HSPA2 antibody 66291-1-IgDownload protocol
IHC protocol for HSPA2 antibody 66291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Clin Epigenetics

Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations.

Authors - J Pei

Front Cardiovasc Med

Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy.

Authors - Maike Schuldt


Protein Quality Control Activation and Microtubule Remodeling in Hypertrophic Cardiomyopathy.

Authors - Larissa M Dorsch