HSPA2 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP00465-1

  The mean HSPA2 concentration was determined to be 34.57 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00465-1

  Sandwich ELISA standard curve of MP00465-1, Human HSPA2 Recombinant Matched Antibody Pair - PBS only. 83466-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3539. 83466-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Cytometric bead array standard curve of MP00465-1

  Cytometric bead array standard curve of MP00465-1, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-3-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83466-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83466-3-RR against Human HSPA2 were performed. The affinity constant is 1.28 nM.

HSPA2 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 83466-3-PBS

Clone No.240421A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Heat shock 70 kDa protein 2, Heat shock protein family A member 2, Heat shock-related 70 kDa protein 2, HSP70 2

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Product Information

83466-3-PBS targets HSPA2 as part of a matched antibody pair:

MP00465-1: 83466-1-PBS capture and 83466-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3539

  Product name: Recombinant human HSPA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 290-639 aa of BC036107

  Sequence: LYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRNTTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEEVD

• Full Name: heat shock 70kDa protein 2
• Calculated Molecular Weight: 639 aa, 70 kDa
• GenBank Accession Number: BC036107
• Gene Symbol: HSPA2
• Gene ID (NCBI): 3306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54652
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.