HSPA4L Polyclonal antibody, PBS Only

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• HSPA4L Polyclonal antibody, PBS Only (32468-1-PBS)

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  WB analysis using 32468-1-AP (same clone as 32468-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32468-1-AP (HSPA4L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 32468-1-AP (same clone as 32468-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 32468-1-AP (same clone as 32468-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 32468-1-AP (same clone as 32468-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32468-1-PBS in a different storage buffer formulation.

HSPA4L Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 32468-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

APG1, Heat shock 70 kDa protein 4L, Heat shock 70-related protein APG-1, Heat shock protein family H member 3, Heat-shock protein family A member 4-like protein

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Product Information

32468-1-PBS targets HSPA4L in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38354

  Product name: Recombinant human HSPA4L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 690-839 aa of NM_001317381.1

  Sequence: MKYMEHEERPKALNDLGKKIQLVMKVIEAYRNKDERYDHLDPTEMEKVEKCISDAMSWLNSKMNAQNKLSLTQDPVVKVSEIVAKSKELDNFCNPIIYKPKPKAEVPEDKPKANSEHNGPMDGQSGTETKSDSTKDSSQHTKSSGEMEVD*

• Full Name: heat shock 70kDa protein 4-like
• Calculated Molecular Weight: 95kDa，839aa
• Observed Molecular Weight: 95~100 kDa
• GenBank Accession Number: NM_001317381.1
• Gene Symbol: HSPA4L
• Gene ID (NCBI): 22824
• RRID: AB_3742620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95757
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HSPA4L (Heat shock 70-kDa protein 4-like), also known as APG1 or OSP94, is a member of the heat shock protein 110 family, whose mRNA is strongly expressed in normal human testis and overexpressed in leukemia cells. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells. Up-regulation of the Hspa4l gene in kidney and renal cell lines by osmotic stress suggested that Hspa4l may function to enable the kidney to compensate for osmotic stress present within the kidney (PMID: 16923965, 17588478).