Tested Applications
| Positive WB detected in | HL-60 cells, MCF-7 cells, U-937 cells |
| Positive IHC detected in | mouse lung tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13616-1-AP targets HSPA6 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4522 Product name: Recombinant human HSPA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 294-643 aa of BC035665 Sequence: DFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTYSDNQPGVFIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILSVTATDRSTGKANKITITNDKGRLSKEEVERMVHEAEQYKAEDEAQRDRVAAKNSLEAHVFHVKGSLQEESLRDKIPEEDRRKMQDKCREVLAWLEHNQLAEKEEYEHQKRELEQICRPIFSRLYGGPGVPGGSSCGTQARQGDPSTGPIIEEVD Predict reactive species |
| Full Name | heat shock 70kDa protein 6 (HSP70B') |
| Calculated Molecular Weight | 643 aa, 71 kDa |
| Observed Molecular Weight | 68-71 kDa |
| GenBank Accession Number | BC035665 |
| Gene Symbol | HSPA6 |
| Gene ID (NCBI) | 3310 |
| RRID | AB_2120122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17066 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSPA6 antibody 13616-1-AP | Download protocol |
| WB protocol for HSPA6 antibody 13616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HSPA6 Polyclonal antibody (Cat# 13616-1-AP) has 19 citations. These appear in journals including Journal of Translational Medicine, International Journal of Molecular Medicine, Animal Nutrition, ACS Applied Materials & Interfaces, Cell Reports, Cancer Cell International, Life Sciences, Journal of Cellular and Molecular Medicine, Metabolites, Biochimica et Biophysica Acta, American Journal of Physiology, Journal of Orthopaedic Surgery and Research, Photochemical & Photobiological Sciences, American Journal of Cancer Research, Tissue & Cell, Evidence-Based Complementary and Alternative Medicine, and Bioscience, Biotechnology, and Biochemistry. Research fields span cancer biology, heat shock protein signaling, cell stress, and immunology.
| Species | Application | Title |
|---|---|---|
J Transl Med Exosome-derived Menin from cancer-associated fibroblasts promotes gastric cancer progression by activating the HSPA6/JNK/JunD pathway and inducing EMT. | ||
Int J Mol Med Role of hyperthermia in overcoming HPV‑mediated MHC‑Ⅰ downregulation and promoting immune surveillance. | ||
Anim Nutr Protective impacts of berberine in weaned piglets fed without zinc oxide: Insight of jejunal barrier function, water absorption, and gut microbiota and metabolism. | ||
ACS Appl Mater Interfaces Electrostatic Force-Enabled Microneedle Patches that Exploit Photoredox Catalysis for Transdermal Phototherapy | ||
Cell Rep HSPA6 maintains IMPDH2 phosphorylation to regulate GTP synthesis for driving radioresistance of glioblastoma stem cells.
| ||
Cell Rep NFE2-driven neutrophil polarization promotes pancreatic cancer liver metastasis progression |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The user performed Western Blot on MDA-MB-231 cells at a 1:1000 dilution and reported that the antibody produced a very clean band with no background signal, indicating strong specificity and sensitivity in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Laureano (Verified Customer) (12-26-2019) | The antibody gave a very clean band without background
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