HSPB8 Polyclonal antibody
HSPB8 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, CoIP, ELISA
Cat no : 15287-1-AP
Validation Data Gallery
|Positive WB detected in||Neuro-2a cells, SGC-7901 cells, mouse brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue|
|Positive IHC detected in||human heart tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 4 publications below|
|IHC||See 2 publications below|
|IF||See 1 publications below|
|CoIP||See 1 publications below|
15287-1-AP targets HSPB8 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSPB8 fusion protein Ag7456|
|Full Name||heat shock 22kDa protein 8|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||20-22 kDa|
|GenBank accession number||BC002673|
|Gene ID (NCBI)||26353|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for HSPB8 antibody 15287-1-AP||Download protocol|
|IHC protocol for HSPB8 antibody 15287-1-AP||Download protocol|
|IF protocol for HSPB8 antibody 15287-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.
The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.
J Cell Biochem
Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes.
BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.
Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.
Biochem Cell Biol
HSPB8 facilitates prostate cancer progression via activating the JAK/STAT3 signaling pathway