HSPBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSPBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10211-1-AP

Synonyms

Hsp70-binding protein 1, Hsp70 interacting protein 2, Hsp70 interacting protein 1, Hsp70 binding protein 2, Hsp70 binding protein 1

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Validation Data Gallery

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  WB analysis using 10211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10211-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10211-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10211-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10211-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10211-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10211-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10211-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10211-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10211-1-AP (HSPBP1 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of HeLa using 10211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPBP1 antibody (10211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, human heart tissue, human skeletal muscle tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

10211-1-AP targets HSPBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPBP1 fusion protein Ag0282
• Full Name: HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC002373
• Gene Symbol: HSPBP1
• Gene ID (NCBI): 23640
• RRID: AB_2121242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hsp70-interacting protein (HSPBP1)is a protein of approximately 40 kilodaltons detected in cell extracts. Its mRNA is present in all tissues analyzed but is most abundant in heart and skeletal muscle. HSPBP1 binds the ATPase domain of Hsp70 and inhibits approximately 90% of the Hsp40-activated Hsp70 ATPase activity by preventing ATP binding to Hsp70.

Publications

Species	Application	Title
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
human	WB	Mol Immunol; HSPBP1 facilitates cellular RLR-mediated antiviral response by inhibiting the K48-linked ubiquitination of RIG-I.; Authors - Ya-Xian Yang ; KD Validated