HSP60 Antibody

HSP60 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F10E7

Cat no : 66041-1-Ig

Synonyms

Heat shock protein 60, HSP 60, HSP60, HSP65, HSPD1



Tested Applications

Positive WB detected inROS1728 cells, HeLa cells, HEK-293 cells, Jurkat cells, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman ovary tumor tissue, human skin cancer tissue, human spleen tissue, human tonsillitis tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells, A549 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 66041-1-Ig immunogen is Fusion Protein HSP60 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7848
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession number BC003030
Gene symbol HSPD1
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
WB protocol for HSP60 antibody 66041-1-IgDownload protocol
IHC protocol for HSP60 antibody 66041-1-IgDownload protocol
IF protocol for HSP60 antibody 66041-1-IgDownload protocol
IP protocol for HSP60 antibody 66041-1-IgDownload protocol
FC protocol for HSP60 antibody 66041-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis.

Authors - Ping Wang
ratWB, IF

Neurotoxicology

Tetrachlorobenzoquinone triggers the cleavage of Bid and promotes the cross-talk of extrinsic and intrinsic apoptotic signalings in pheochromocytoma (PC) 12 cells.

Authors - Lihua Hu
ratIF

J Neuroinflammation

DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke.

Authors - Li Peng
humanWB

Mol Cancer

BMI1, a new target of CK2α.

Authors - Soumyajit Banerjee Mustafi
humanWB, IF

Nat Immunol

Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.

Authors - Jing Geng